Topic 2.3 Nucleic Acid Hybridization & Expression Analysis

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In this topic you’ll learn about Gene Expression, Nucleic Acid Labeling, Blotting Techniques, In-Situ Hybridization, and DNA Microarrays. You will start by clicking on the next button at the bottom of the page to being reviewing the basics elements of laboratory safety.



Upon completion of this module topic, you will:

1. be able to explain the various types of blotting techniques (Southern, Northern) for detecting specific DNA and RNA fragments using various types of probes for hybridization (DNA fragment probes, oligonucleotides).

2. be able to explain the significance behind labeling probes and the methods used to label probes (radioactive isotopes, alkaline phosphatase, antibodies, chemiluminescence probes, fluorescence probes).

3. be able to explain methods used to visually detect probes bound to targeted DNA or RNA.

4. be able to explain what DNA Microarray Assays are and how they are used to conduct large scale analyses of gene expression.


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