Objectives

Upon completion of this module topic, you should:

  • be able to set up a Polymerase Chain Reaction (PCR) using DNA samples, primers, buffer, dNTPs, and polymerase enzyme;
  • understand the significance and role of each component in the reaction;
  • be able to load PCR samples into a thermocycler machine under supervision and understand the mechanisms behind the different steps taking place inside of the thermocycler throughout the PCR reaction;
  • have a general understanding of Real-Time PCR and its applications for quantitating gene expression;
  • understand the mechanism of DNA sequencing and the methods implemented for sequencing DNA;