Objectives

Upon completion of this module topic, you will:

  • Be able to set up a Polymerase Chain Reaction (PCR) using DNA samples, primers, buffer, dNTPs, and polymerase enzyme.  You should be able to explain the significance and role of each component in the reaction.
  • Be able to load PCR samples into a thermocycler machine and describe the mechanisms behind the different steps taking place inside of the thermocycler throughout the PCR reaction.
  • Be able to explain Real-Time PCR and the two common methods for detection.
  • Be able to explain the mechanism of DNA sequencing and identify the advantages of using the Dye Termination method.