Objectives

Upon completion of this module topic, you should:

  1. Be able to analyze gene expression resulting from a cell transfection experiment, using luciferase or GFP reporter constructs.
  2. Be able to prepare cell lysates from transfected cells by suspending adherent cells and lysing cells.
  3. Be able to conduct a luciferase assay on cell lysates by carrying out luciferase reactions and using bioluminescence to measure luciferase expression.
  4. Be able to use a luminometer to measure bioluminescence of cell lysate samples.